SQL Express connecting form remote error 26

Dear All

SQL Express installed on our server  ServerA and instance name is SqlExpress which is working fine on the local server, recently we activated windows server after trial expired... then onward we are facing an issue from remote sites.

that from remote SQL Express we are unable to connect database Error showing  SQL Network Interfaces, error: 26 – Error Locating Server/Instance Specified,

the thing is while connecting Sql instance name with port it's working fine but without port it's not connecting

EX:   ServerA\SqlExpress,1433   (it's connecting but our application giving error)

when we try to connect with ServerA\SqlExpress  the connection is failed with the following error


A network-related or instance-specific error occurred while establishing a connection to SQL Server. The server was not found or was not accessible. Verify that the instance name is correct and that SQL Server is configured to allow remote connections. (provider: SQL Network Interfaces, error: 26 - Error Locating Server/Instance Specified) (.Net SqlClient Data Provider)

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For help, click: http://go.microsoft.com/fwlink?ProdName=Microsoft+SQL+Server&EvtSrc=MSSQLServer&EvtID=-1&LinkId=20476

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Error Number: -1
Severity: 20
State: 0


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Program Location:

   at System.Data.SqlClient.SqlInternalConnection.OnError(SqlException exception, Boolean breakConnection)
   at System.Data.SqlClient.TdsParser.ThrowExceptionAndWarning(TdsParserStateObject stateObj)
   at System.Data.SqlClient.TdsParser.Connect(ServerInfo serverInfo, SqlInternalConnectionTds connHandler, Boolean ignoreSniOpenTimeout, Int64 timerExpire, Boolean encrypt, Boolean trustServerCert, Boolean integratedSecurity, SqlConnection owningObject)
   at System.Data.SqlClient.SqlInternalConnectionTds.AttemptOneLogin(ServerInfo serverInfo, String newPassword, Boolean ignoreSniOpenTimeout, Int64 timerExpire, SqlConnection owningObject)
   at System.Data.SqlClient.SqlInternalConnectionTds.LoginNoFailover(String host, String newPassword, Boolean redirectedUserInstance, SqlConnection owningObject, SqlConnectionString connectionOptions, Int64 timerStart)
   at System.Data.SqlClient.SqlInternalConnectionTds.OpenLoginEnlist(SqlConnection owningObject, SqlConnectionString connectionOptions, String newPassword, Boolean redirectedUserInstance)
   at System.Data.SqlClient.SqlInternalConnectionTds..ctor(DbConnectionPoolIdentity identity, SqlConnectionString connectionOptions, Object providerInfo, String newPassword, SqlConnection owningObject, Boolean redirectedUserInstance)
   at System.Data.SqlClient.SqlConnectionFactory.CreateConnection(DbConnectionOptions options, Object poolGroupProviderInfo, DbConnectionPool pool, DbConnection owningConnection)
   at System.Data.ProviderBase.DbConnectionFactory.CreateNonPooledConnection(DbConnection owningConnection, DbConnectionPoolGroup poolGroup)
   at System.Data.ProviderBase.DbConnectionFactory.GetConnection(DbConnection owningConnection)
   at System.Data.ProviderBase.DbConnectionClosed.OpenConnection(DbConnection outerConnection, DbConnectionFactory connectionFactory)
   at System.Data.SqlClient.SqlConnection.Open()
   at Microsoft.SqlServer.Management.SqlStudio.Explorer.ObjectExplorerService.ValidateConnection(UIConnectionInfo ci, IServerType server)
   at Microsoft.SqlServer.Management.UI.ConnectionDlg.Connector.ConnectionThreadUser()

could you please guide me how to connect without the port ...

thanks