Microsoft Remote app to an application server slow

Hello we have a remote app to our server that is a remote desktop that opens up the an application directly.  

We have two types of terminal servers in place that access the same healthcare application.
1. Connection #1  is remote app shortcut on the users desktop that connects to the application server and opens client directly on the server.  We are seeing some slowdown in the user experience as they click around on the application this remote app that directly opens the application on the application server.
2. We have a terminal server that hosts the client and then connects to the application server that is on the same virtual host.  
3. I have compared it  the terminal server experience to the remote app and it is sometime slower.  What can directly affect this remote app experience as I need to troubleshoot this connection?  Would there be any log files that might relate to this?

couple of FYI's
- Server 2008r2
-Virtual environment but running on the same host
-Host has plenty of resources for application server and the TS
-We see this happen periodically
barl009Asked:
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No MoreConnect With a Mentor Commented:
Ping, tracert the connection to servers, I would say, that you have networking issue.

I would also run wireshark, to see whats happening on your network

Check logs on switches and routers / firewall

Test data transfer between client computers and servers
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No MoreCommented:
Hey whats your internal /external network speed ?

Whats your hard disks setup ?

How many VM's are running on Hyper-v Host ?


Are those users accessing this APP from outside, are those issues happening even inside internal network ?
Technical info about server like NIC's  speed / teaming ?
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barl009Author Commented:
-Our network lan speed is 100 mb/sec.  
-Hard disk set up, not sure how to answer the hard disk setup for the VMWARE, but know that our hosts are all over resourced and our the disk on that VM is one C drive that has plenty of space and enough ram.  We watched the use of the resources on the OS side and it looks healthy as well.
-There are 5 VM's on that particular host.   The application server and the Terminal Server on same host.
-Our networking on the networking tab in the VMware is all redundant and is running at 1000
VMWARE-info.docx
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