Permission on folders

Hello All,

Below script worked fine. Now i have added two more line to add the permission to folder. after folder created permission will be assigned only last line of permission I.E domain\userread123.  First 02 permission lines not assigning permission,

Thanks in advance

Set-Location "C:\Users\narasimha.s\Desktop\foldertest"
$Folders = Import-Csv C:\Users\Narasimha.s\Desktop\foldertest\project.csv
ForEach ($Folder in $Folders) {
    $newFolder = New-Item -name $Folder.Name -type directory
    $acl = Get-Acl $newFolder.FullName

    $ace = New-Object System.Security.AccessControl.FileSystemAccessRule('domain\usermodify', 'Modify,Synchronize', 'ContainerInherit,ObjectInherit', 'None', 'Allow')
    $ace = New-Object System.Security.AccessControl.FileSystemAccessRule('domain\userread', 'Read,Synchronize', 'ContainerInherit,ObjectInherit', 'None', 'Allow')
    $ace = New-Object System.Security.AccessControl.FileSystemAccessRule('domain\userread123', 'Read,Synchronize', 'ContainerInherit,ObjectInherit', 'None', 'Allow')
    $acl.AddAccessRule($ace)

    Set-Acl $newFolder.FullName -AclObject $acl

}

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