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According to Skype no. You can only call Skype for Business users.
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Our business has office 365 and skype for business. Can I add a regular external skype user to my skype for business and communicate like any other internal user?
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Our business has office 365 and skype for business. Can I add a regular external skype user to my skype for business and communicate like any other internal user?

Can office 365 skype for business users contact regular skype users

Office 365 is a group of software plus services subscriptions that provides productivity software and related services to its subscribers. Office 365 allows the use of Microsoft Office apps on Windows and OS X, provides storage space on Microsoft's cloud storage service OneDrive, and grants 60 Skype minutes per month. Office 365 includes e-mail and social networking services through hosted versions of Exchange Server, Skype for Business Server, SharePoint and Office Online, integration with Yammer, as well as access to the Office software. All of Office 365's components can be managed and configured through an online portal; users can be added manually, imported from a CSV file, or Office 365 can be set up for single sign-on with a local Active Directory using Active Directory Federation Services.