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2 FA on a Windows server folder

We have a request to put the highest possible security on a folder.  We've been asked to put 2 FA on access, but only to those who need to access that folder and preferably only when they have to access that folder.

We can't see how this is directly possible using Duo or RSA, but if there is we'd love to know how.  If it is not, what kind of "out of the box" ways can we have a very high security folder within an environment?

We considered moving it to the cloud to secure it with 2FA, but this also then exposes a cloud/Internet component and they are looking for the tightest security possible.

Thanks to everyone who contributes!
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Dmitri Farafontov
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I saw this as well for applications, but I didn't see anything where it would apply for file access to a particular folder?
Looks like this can't easily be done at this time.