Ok so over this week I removed my last 2008 DC and moved to 2012 R2, everything went as planned no errors or anything. However today my remote users aren't able to access their published RemoteApps. They can browse to the gateway authenticate fine and see their apps, but when they try to open them 2 odd things happened. It times out and prompts them for their user, then just keeps doing that to no avail.
2 other clues as to what it may be. They also get a prompt to trust the certificate, which they shouldn't as the rds deployment uses a properly signed one, and this had been working fine for 6 months now. The other oddity is my CiscoAnyConnect won't authenticate to the LDAP properly either. If i add them as a AAA local user they can vpn then they can log in, but they've never had to VPN to access this before the DC change.