Problem with R Studio - 2 levels only

I ran into a problem in R Studio. I am trying to run a t-test and it says "grouping data must have exactly 2 levels". Anyone know how to do this t-test with our values? We are trying to use bar charts showing means with error bars by presenting the results in Excel. Also need to know how to segment results using ANOVA.
Taylor ConranMarketing and Community Relations InternAsked:
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NorieAnalyst Assistant Commented:

What do you see if you view the data  after running na.omit on it?

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Taylor ConranMarketing and Community Relations InternAuthor Commented:
Figured it out on my own but thanks! Full points to you.
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